Test No. 302C: Inherent Biodegradability: Modified MITI Test (II)
This Test Guideline describes the modified MITI test (II). This test permits the measurement
of the Biochemical Oxygen Demand (BOD) and the analysis of residual chemicals in order
to evaluate the inherent biodegradability of chemical substances which have been found
by the Standard MITI Method (I) to be low degradable.
An automated closed-system oxygen consumption measuring apparatus (BOD-meter) is used.
Chemicals to be tested are inoculated in the testing vessels (six bottles with different
quantities of test chemical) with micro-organisms. In order to check the activity
of the inoculum, the use of control substances (aniline, sodium acetate or sodium
benzoate) is desirable. During the test period, the BOD is measured continuously.
Biodegradability is calculated on the basis of BOD and supplemental chemical analysis,
such as measurement of the dissolved organic carbon concentration, concentration of
residual chemicals, etc. The BOD curve is obtained continuously and automatically
for 14 to 28 days. After the 14 to 28 days of testing, pH, residual chemicals and
intermediates in the testing vessels are analysed.
Published on September 08, 2009Also available in: French
In series:OECD Guidelines for the Testing of Chemicals, Section 3: Environmental fate and behaviourview more titles