Test No. 240: Medaka Extended One Generation Reproduction Test (MEOGRT)
This Test Guideline describes the Medaka Extended One Generation Test (MEOGRT), which
exposes fish over multiple generations to give data relevant to ecological hazard
and risk assessment of chemicals, including suspected endocrine disrupting chemicals
(EDCs). Exposure in the MEOGRT starts with spawning fish (P or F0 generation) and
continues until hatching (until two weeks post fertilization, wpf) in the second (F2)
generation. This Test Guideline measures several biological endpoints. Primary emphasis
is given to potential adverse effects on population relevant parameters including
survival, gross development, growth and reproduction (fecundity). Secondarily, in
order to provide mechanistic information and provide linkage between results from
other kinds of field and laboratory studies, where there is a posteriori evidence
for a chemical having potential endocrine disrupter activity (e.g. androgenic or oestrogenic
activity in other tests and assays) then other useful information is obtained by measuring
vitellogenin (vtg) mRNA (or vitellogenin protein, VTG), phenotypic secondary sex characteristics
(SSC) as related to genetic sex, and evaluating histopathology.
Published on July 04, 2023Also available in: French
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